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Vliv alternativních zdrojů bílkovin v rybím krmivu na růst ryb a rostlin ve dvousmyčkovém akvaponickém systému
NIKL, Ondřej
The objective of this thesis was to evaluate the production efficiency of feeds which were designed with the intention of developing a special aquaponic feed. Further, to evaluate the content of nutrients in water, sludge and nutrient solutions and their influence on plant growth and finally suggest possible modifications to the feed composition. Aquaponic feed should respect nutritional requirements of fishes and plants, not put burden on the environment by not used nutrients and with regards to sustainability, use alternative sources of protein instead of marine fish meal. Because of that, three experimental isoprotein and isocaloric feeds were proposed. Feed A contained conventional ingredients including marine fish meal. Feed B used freshwater fish meal and exclusively plant proteins not regarding its origin. Feed C was made of sustainable and local sources of protein. The experiment was conducted in the aquaponic hall of Faculty of Fisheries and Protection of Waters, University of South Bohemia. As experimental material was used Nile tilapia (Oreochromis niloticus) and lettuce (Lactuca sativa). Feed B shown similar values of all production indicators as conventional feed A. Weight of fishes in system A after thirteen weeks increased by 143,68 % and in system B by 136,56 %. Weight of fishes in system B was at the end of experiment inconclusively lower by 4,08 %. Feed conversion ratio (FCR) of feed B (1,92?0,10) was inconclusively higher by 8,47 % against FCR of feed A (1,77?0,13). Average weight of aerial parts of plants growing in nutrient solution created from mineralized sludge gathered from system B, supplemented with missing nutrients reached value 221,8?33,9 g after four weeks. That was inconclusively more by 44,21 % than average weight of plants grown in special hydroponic solution and by 8,62 % more than weight of plants grown in enriched solution gathered from system A. Experimentally it was proven that in aquaponic feeds it is possible to substitute marine fish meal by alternative source of protein and using these feeds ensure an effective nutrition for fish and plants in a two-loop aquaponic system.
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Využití hmyzí moučky z potemníka moučného (Tenebrio molitor) v krmivech pro okouna říčního (Perca fluviatilis L.).
VOLSKÝ, Jan
Teoreical part of this study focused on biology, importance and breeding of perch and types of insect, that EU approved for using in feed of animal, that are intended for human consuption. At the end of theretical part was described using of insect in fish feed. Practical part of our experiment consist of two tests. The rearing part of the experiment aimed to evaluate the effect of the graded amount of insect meal from Tenebrio molitor on biometrical parameters and biochemical indicators of perch (Perca fluviatilis L.). The swimming performance test aimed to assess the effect of experimental diets on the physiology of fish. The subject of the diploma thesis was also to compare the influence of diets on environmental impacts. For this purpose, 4 experimental diets were compiled. The diets differed from each other by substituting fish meal by insect meal. In the control group (TM0) 100 % of animal protein consist of fish meal. In other diets were fish meal replaced by 25 % (TM25), 50 % (TM50) and 75 % (TM75) meal of T. molitor. Each tested group had 4 replicates. The rearing part of the experiment lasted 119 days. At the end of the feeding trial, 80 fish (20 from each group) were selected for swimming performance tests.
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Možnosti využití hmyzí moučky v krmivech pro candáta obecného (Sander lucioperca)
KODRAS, Marek
A feeding trial was carried out to assess the effect of replacing fish meal by partially deffated black soldier fly pre-pupae meal in feed for pikeperch (Sander lucioperca). A diet with fish meal, that covered 30 % of total protein, was used as a control. Three other diets contained 7,5%, 15% and 30% of insect meal, which coresponded to 25% (H25), 50% (H50) and 100% (H100) replacement of fish meal. Each tested group had 3 repetitions and experiment lasted 63 days. At the end of the trial, groups H25 and H50 did not show any significant statistical differences in growth and lenght performance compared to CON. There were no differences among groups in coefficient of variation, condition factor, hematocrit, feed conversion ratio, economic conversion ratio, fat and ash content in muscle. On the other hand, significant differences were observed in specific growth rate, contain of proteins in muscles, dry matter, somatic indexes and economic profit index. No differences in survival were found until 42. day. However, between 42. a 63. day of rearing high mortality caused by bacterial infection was observed in two of three tanks fed with H100 diet. This fact influenced this parameter for group H100. In conclusion, 7,5 % inclusion of insect meal from partially deffated black soldier fly pre-pupae meal, corresponding to 25% replacement of fish meal, may successfully replace fish meal in diet for pikeperch, as this substitution did not have negative influence on any parameter.
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Porovnání účinnosti peletovaného krmiva s obsahem hmyzu a směsi s běžným složením při odchovu okouna říčního (Perca fluviatilis) v poloprovozních podmínkách
NOVOTNÝ, Martin
This thesis deals with the possibility of using insects as a potential source of proteins in artificial feed for Eurasian perch. In conventional feed, 25 % of fish meal was replaced by meal from adult Acheta domestica and Zophobas morio. There were performed two 12-week long experiments, the first one in the Experimental Fishery Workplace at the Faculty of Fisheries and Water Protection of the University of South Bohemia in Vodňany and the second one in the commercial operation of Anapartners s.r.o. Company in Prague Horní Počernice. The production indicators of the breed were evaluated and the fat and fatty acids in the fish muscles were analyzed as an important indicator of its nutritional value. At the end of both experiments, fish fed by experimental feeds showed lower length and weight gains and worse feed conversion, compared to fish fed by control diet. During the experiment and after its completion, the feed effect on fish survival were not noticed. Furthermore, there were not found any changes in fat content in fish muscles, but changes in the content of individual fatty acids were noticed. In fish fed with experimental feed, the content of linoleic acid (C18: 2n-6) increased as well as the total content of n-6 fatty acids, on the contrary, the content of n-3 fatty acids in the fish muscle decreased more than in fish fed by control diet. This also affected the resulting ratio of the n-6 fatty acid to n-3 fatty acid ratio, while this ratio increased significantly in the experimental fish group. Changes in fatty acids content in fish muscle were small and did not affect its nutritional value from the perspective of human consumption. In other experiments dealing with this topic, it is necessary to focus on testing of other insect species or to modify the quantity of substituted fishmeal.
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